Bioactivity Evaluation

Systematic bioactivity evaluation of potential antibacterial agents in vitro: From biosensor-based HTS to follow-up studies (WP3)

Dr. Päivi Tammela
University of Helsinki

This project involves two related components: (i) Validation of biosensors for HTS and HTS campaigns. Biosensor strains supplied by partners will be adapted to HTS format, optimized and validated for HTS. The HTS platform will be used for screening the compound libraries provided by WP2. (ii) Follow-up studies in vitro. The most promising hits from HTS will be subjected to (a) activity profiling using a panel of selected clinical strains according to CLSI guidelines, (b) selectivity testing on mammalian cell lines, and (c) activity testing in host-pathogen co-cultures of bacteria and animal cells (mimicking in vivo infection).

Objectives: To develop biosensor-based HTS platform and to show its functionality in antibacterial screening. To evaluate in vitro antibacterial properties of compounds provided by WP1-2 and to further validate the HTS hits through a set of in vitro follow-up studies.

Expected Results: Proven functionality of biosensor-based HTS platform in detecting antibacterial properties. Identification of novel antibacterial compounds against Gram-negative bacteria, which show selectivity and promising therapeutic potential.

This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska Curie grant agreement No  642620
European Union